Based on his observations, Henking hypothesized that this extra chromosome, the X element, must play some role in determining the sex of insects. How did researchers discover these so-called sex chromosomes? The variety of sex determination systems. The variety of inheritance patterns described in this article illustrate that sex determination is a complex and varied feature among organisms. Key Questions How can environmental conditions determine sex in some animals? In this case, two of the four quadrants contain the genotype XX, resulting in female insects; the remaining two quadrants contain the genotype X-, resulting in male insects. More than a decade after Henking's work, Nettie Stevens surveyed multiple beetle species and examined the inheritance patterns of their chromosomes.
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